Ikhithi yoVavanyo oluKhawulezayo lwe-COVID-19 (iGolide eyiColloidal)

Ikhithi yoVavanyo oluKhawulezayo lwe-COVID-19 (i-Colloidal Gold) Umfanekiso okhoyo

Inkcazelo emfutshane:

Iinkcukacha zeMveliso

Injongo yokuSebenzisa:

◆Ukufumana izilwa-buhlungu ezingathinteliyo.

◆COVID-19 Ikhithi yoVavanyo oluKhawulezayo lwe-Antibody ye-COVID-19 (i-Colloidal Gold) luvavanyo oluthe gqolo lokugobhoza igazi olujonge ukufunyaniswa okusemgangathweni kwe-antibody ye-SARS-CoV-2 kwigazi lonke lomntu, i-serum okanye iplasma njengoncedo kumanqanaba oVavanyo lwe-anti yabantu. -inoveli ye-coronavirus ethambisa i-antibody titer.

Indlela yesampulu

◆Igazi Elipheleleyo, iSerum, iPlasma

UmGaqo wokuSebenza:

◆Le kit isebenzisa i-immunochromatography.Ikhadi lovavanyo liqulathe:1)i-colloidal ebhalwe ngegolide ebhalwe ngokutsha i-coronavirus S-RBD antigen kunye nolawulo lomgangatho weempawu zegolide ze-antibody;2) umgca omnye wokufumanisa (umgca we-T) kunye nomgca omnye wokulawula umgangatho (umgca weC) we-nitrocellulose membrane.Umgca we-T awushukumi kunye neprotein ye-ACE2 yoMntu yokubhaqa inoveli ye-coronavirus engathathi hlangothi kwaye umgca we-C awushukumi kunye ne-antibody yokulawula umgangatho.

◆ Xa isixa esifanelekileyo sesampuli yovavanyo yongezwa kwisampula yomngxuma wekhadi lokuvavanya, isampuli iya kuqhubela phambili kunye nekhadi lokuvavanya phantsi kwesenzo se-capillary.Ukuba isampuli iqulethe inoveli i-coronavirus engathathi hlangothi, i-antibody iya kuzibophelela kwi-colloidal yegolide ebhalwe inoveli ye-coronavirus antigen.Enye i-antigen yegolide esele ibhalwe ngegolide kwi-immune complex iya kubanjwa yiprotein ye-ACE2 engashukumiyo.

i-membrane ukwenza umgca we-T omfusa-obomvu, ukuqina komgca we-t Ngokuphambene ngokuhambelana nokuxinana kwe-antibody.

◆Ikhadi lokuvavanya kwakhona liqulethe umgca wokulawula umgangatho C .Umgca wokulawula umgangatho we-fuchsia C kufuneka ubonakale kungakhathaliseki ukuba umgca wokuvavanya uvela.Ukuba umgca wokulawula umgangatho we-C awubonakali, umphumo wokuvavanya awusebenzi, kwaye isampuli kufuneka ihlolwe kwakhona kunye nelinye ikhadi lokuvavanya.

Iinkcukacha zemveliso:

◆Severe acute breathing syndrome coronavirus 2 (SARS-CoV-2, okanye 2019-nCoV) yintsholongwane egutyungelweyo ye-RNA engacandekiyo.Yeyona

unobangela we-COVID-19, eyosulela ebantwini.

◆SARS-CoV-2 ineprotein ezininzi zolwakhiwo eziquka i-spike (S), imvulophu (E), inwebu (M) kunye ne-nucleocapsid (N).Iprotheni ye-spike (S) iqulethe i-domain binding domain (RBD), ejongene nokuqaphela i-cell surface receptor, i-antigens ekuguquleni i-enzyme-2 (ACE2).Ifunyenwe i-uman ACE2 receptor ekhokelela kwi-endocytosis kwiiseli zemiphunga enzulu kunye nokuphindaphinda kwentsholongwane.

◆Ukosuleleka ngogonyo lwe-SARS-CoV-2 okanye lwe-SARS-COV-2 logonyo luqalisa impendulo yomzimba yokuvelisa izilwa-buhlungu ezibonelela ngokhuseleko kusulelo lwexesha elizayo kwiintsholongwane.Izilwa-buhlungu ezithi zijolise kumkhosi we-ACE2 we-receptor-binding domain (RBD) ye-SAR-COV-2 spike protein ibonisa isithembiso sonyango kunye nokusebenza kakuhle ngokhuseleko.

◆ISerum okanye iplasma specimen/incam yegazi yegazi.

◆Ukufumana umyinge we-semi-quantitative ye-antibody engathathi hlangothi.

◆ Uvavanyo lwe-antibody olungathathi hlangothi lunokubona ukuba ngaba kukho izilwa-buhlungu ezingathathi hlangothi ngokuchasene ne-SARS-CoV-2 emzimbeni.

◆Nceda ukulandelela ubomi obude bokhuselo lomzimba emva kokugonywa.