Porcine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) yiporcine enteric pathogenic coronavirus esandula ukufunyanwa enokubangela urhudo olunamanzi kumantshontsho asandula kuzalwa kwaye ibangele ilahleko enkulu yezoqoqosho kwishishini lehagu.Okwangoku, akukho ndlela ifanelekileyo ye-serological yokuvavanya ukusebenza kosulelo lwe-SADS-CoV kunye nesitofu sokugonya, ngoko kukho imfuneko engxamisekileyo yokusebenzisa i-enzyme-linked immunosorbent assay (ELISA) ukwenza oku kusilela.Apha, i-plasmid ephinda iphinda iveze i-SADS-CoV spike (S) protein edityaniswe nedomeyini yomntu ye-IgG Fc yakhelwe ukuvelisa i-baculovirus ephinda iphinde yavezwa kwiiseli ze-HEK 293F.Iprotheni ye-S-Fc ihlanjululwa ngeprotheyini ye-G resin kwaye igcina i-reactivity kunye ne-anti-human Fc kunye ne-anti-SADS-CoV antibodies.Emva koko iprotheni ye-S-Fc yayisetyenziselwa ukuphuhlisa i-ELISA engathanga ngqo (S-iELISA) kunye nokwandisa iimeko zokusabela ze-S-iELISA.Ngenxa yoko, ngokuhlalutya ixabiso le-OD450nm le-40 ye-SADS-CoV i-sera engalunganga eqinisekisiwe yi-immunofluorescence assay (IFA) kunye ne-Western blotting, ixabiso lokunqunyulwa linqunywe ukuba libe yi-0.3711.I-coefficient of variation (CV) ye-6 SADS-CoV positive sera ngaphakathi naphakathi kokuqhutywa kwe-S-iELISA zonke bezingaphantsi kwe-10%.Uvavanyo lwe-cross-reactivity lubonise ukuba i-S-iELISA ayinayo i-cross-reactivity kunye nenye i-sera ye-porcine virus.Ukongezelela, ngokusekelwe ekufumaneni iisampuli ze-serum zeklinikhi ze-111, izinga lokuhambelana kwe-IFA kunye ne-S-iELISA yayingu-97.3%.Uvavanyo lokungathathi hlangothi kwentsholongwane kunye ne-7 eyahlukeneyo ye-OD450nm yamaxabiso e-serum ibonise ukuba ixabiso le-OD450nm elifunyenwe yi-S-iELISA lalinxibelelene ngokuqinisekileyo novavanyo lwe-virus.Ekugqibeleni, i-S-iELISA yenziwa kwiisampulu zeserum zefama yeehagu ezingama-300.Iikiti zorhwebo zezinye i-porcine enteroviruses zibonise ukuba i-IgG imilinganiselo emihle ye-SADS-CoV, TGEV, PDCoV kunye ne-PEDV yayiyi-81.7%, 54%, kunye ne-65.3%, ngokulandelanayo., 6%, ngokulandelanayo.Iziphumo zibonisa ukuba i-S-iELISA icacile, inobuntununtunu, kwaye iyakwazi ukuveliswa, kwaye ingasetyenziselwa ukubona usulelo lwe-SADS-CoV kwishishini lehagu.Eli nqaku likhuselwe yi-copyright.onke Amalungelo Agciniwe.
Ixesha lokuposa: Jun-22-2021